Coding

Part:BBa_K1680007:Design

Designed by: Katharina Sporbeck   Group: iGEM15_Tuebingen   (2015-09-17)

Cre recombinase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 437


Design Notes

The Part is intended for use with the RFC25 standard. Because this standard already contains both a start and a stop codon in the prefix/suffix, the part only sequence does NOT contain these.


Source

The original sequence of Cre recombinase can be found in uniprot [http://www.uniprot.org/uniprot/P06956]. This sequence was then codon optimized for expression in Saccharomyces cerevisiae and compability with RFC25 standard by Team Tuebingen.

References