Coding
Part:BBa_K1680007:Design
Designed by: Katharina Sporbeck Group: iGEM15_Tuebingen (2015-09-17)
Cre recombinase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 437
Design Notes
The Part is intended for use with the RFC25 standard. Because this standard already contains both a start and a stop codon in the prefix/suffix, the part only sequence does NOT contain these.
Source
The original sequence of Cre recombinase can be found in uniprot [http://www.uniprot.org/uniprot/P06956]. This sequence was then codon optimized for expression in Saccharomyces cerevisiae and compability with RFC25 standard by Team Tuebingen.